A bottle of luminol.

Ema's glasses

A pair of tinted glasses used to view reacting luminol.

Luminol is a chemical known for its chemiluminescence. When it reacts with blood, it glows a vivid blue; as such, it is used in crime scene investigations to find traces of blood that would otherwise be hidden.

Bruce Goodman's murderEdit

Main article: Rise from the Ashes

While investigating the murder of Bruce Goodman, Phoenix Wright was given a bottle of luminol by Ema Skye to search for traces of blood in a number of locations, including the police department's evidence room and the underground parking lot of the prosecutor's office. The results proved to be very helpful, as Wright found the bloody handprint of Jake Marshall on one of the lockers in the evidence room.

Tsubasa Kagome's murderEdit

Main article: The Forgotten Turnabout
This article contains information about Ace Attorney media that has been released only in Japan.
The information in this article comes from a game, demo, or other media that has been released in Japan, but not in any predominantly English-speaking country. The subject of this article has not been officially revealed for English versions of this media. English versions of this content are only available through unofficial translations, and names from these unofficial translations are not being used. More information on this can be found here.

If you have personal experience with the item of media in question, you can help the Ace Attorney Wiki by improving on this article. Please heed the manual of style when adding information.

While investigating the murder of Tsubasa Kagome, Miles Edgeworth met Skye on the Big Tower observation deck. When he deduced that the murder may have occurred in a hidden evidence room on the 51st floor, Skye gave him a bottle of luminol to search for bloodstains. Positive results on the ladder leading into the room, a chained chest, and the lift led Edgeworth to deduce that the victim's body had been hidden in the chest before then being transported via the elevator.

Murder of an Interpol agentEdit

Main article: Turnabout Serenade

An example of a positive luminol reaction.

Upon discovering the body of Romein LeTouse in a Sunshine Coliseum dressing room, it was found that the victim had written something in his own blood on the floor, which the killer had then wiped until it was illegible. Ema Skye (now an adult and police detective) gave Apollo Justice the task of testing the blood traces with luminol to see what the victim wrote. Upon applying the substance, the writing turned out to be not the killer's name, as was first thought. Instead it was a series of numbers and letters: IPXX314206. This was soon revealed to be an Interpol ID number, leading to the discovery that the victim had been an undercover agent of said organization; a vital clue that helped Justice to solve the case, clear his client's name, and catch the real murderer.

Investigation at an aquariumEdit

Main article: Turnabout Reclaimed

Luminol was also used by Phoenix Wright, Pearl Fey, and Athena Cykes during the investigation into the murder of Jack Shipley at Shipshape Aquarium. The presence of a bloodstain on the skull rock decoration in the orca pool helped Wright determine that the victim had hit the rock upon falling to his death. After Fey accidentally sprayed some luminol on the aquarium's trained orca, Orla Shipley, and discovered traces of the victim's blood, Wright was led to the conclusion that the orca had been present when Shipley fell.