Each game has a "Cross-Examination ~ Moderato" (尋問 ～モデラート) theme and a "Cross-Examination ~ Allegro" (尋問 ～アレグロ) theme which play during witness testimonies and cross-examinations. In each game, the "Allegro" theme is a faster, more intense and usually extended remix of the "Moderato" theme; "Moderato" plays during earlier testimonies and "Allegro" during later testimonies, when the trial chapter is approaching its climax.
In Apollo Justice: Ace Attorney, a third variation of the local "Cross-Examination" theme is played when Apollo Justice uses his bracelet: "Perceive ~ Surge, Eyes" (みぬく ～ほとばしれ、視線). Ace Attorney Investigations: Miles Edgeworth also had a third variation, "Presto", which is typically used for the final testimony in each case, with two exceptions. This theme is used again in Gyakuten Kenji 2, albeit this time with four exceptions.
- "Cross-Examination ~ Moderato 2007"
- "Cross-Examination ~ Allegro 2007"
- "Perceive ~ Surge, Eyes"
- "Cross-Examination ~ Moderato 2001" - Turnabout Succession only
- "Cross-Examination ~ Allegro 2001" - Turnabout Succession only
- "Cross-Examination ~ Moderato (English Turnabout Mix)" - English Turnabout only
- "Cross-Examination ~ Allegro (English Turnabout Mix)" - English Turnabout only
- "Mass Inquisition ~ Moderato"
- "Mass Inquisition ~ Allegro"
- "Professor Layton vs. Phoenix Wright: Ace Attorney - Ending Theme" - Contains the melody of "Cross-Examination 2001", as well as other tracks including "Professor Layton's Theme", "Objection! 2001" and "Turnabout Sisters Ballad".
The themes "Logic Chess ~ Opening" and "Logic Chess ~ Endgame" from Gyakuten Kenji 2, "Mood Matrix ~ Commence the Psychoanalytical Analysis!" and "Running Wild- Mood Matrix ~ Get a Grip on Yourself!" from Phoenix Wright: Ace Attorney: Dual Destinies and Phoenix Wright: Ace Attorney: Spirit of Justice, and "Closing Argument" from Dai Gyakuten Saiban: Naruhodō Ryūnosuke no Bōken are all thematically similar to the Cross-Examination themes, with the former two have moderato and allegro variations, and the latter one playing when examining the jury's statements similar to a group cross-examination.
Moderato, allegro, and presto are all terms used in musical scores to describe at what tempo (speed) the music should be played; moderato for a moderate tempo, allegro for a quick/lively tempo, and presto for a very quick tempo. Each "Cross-Examination" theme in the Ace Attorney games follow their respective tempos.